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Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

 详情说明

Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit Summary

Catalog         Size / Price
MJE00           1 Kit (2 Plates) / Please Inquire
SMJE00         1 Pack (6 Plates) / Please Inquire

PMJE00         1 Pack (50 Plates) / Please Inquire

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL)
Sensitivity
2 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse and rat MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat CCL2/MCP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-1 in mouse or rat cell culture supernates and serum. It contains E. coli-expressed mouse MCP-1 and antibodies raised against the recombinant factor. This Immunoassay has been shown to accurately quantitate recombinant mouse MCP-1. Results obtained using natural mouse or rat MCP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural MCP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 47.1 311 768 43.6 310 783
Standard Deviation 3.9 15.8 40.6 3.2 14.3 42.8
CV% 8.3 5.1 5.3 7.3 4.6 5.5

Recovery

The recovery of MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=6) 104 91-119
Mouse Serum (n=9) 99 89-113
Rat Cell Culture Supernates (n=4) 109 103-115
Rat Serum (n=4) 109 103-116
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