Human sVCAM-1/CD106 Quantikine ELISA Kit
Human sVCAM-1/CD106 Quantikine ELISA Kit
Human sVCAM-1/CD106 Quantikine ELISA Kit Summary
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货号：DVC00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
6.3 - 200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human sVCAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human sVCAM-1 Immunoassay is a 2 hour solid-phase ELISA designed to measure human sVCAM-1 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sVCAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sVCAM-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
583
|
1423
|
2421
|
670
|
1571
|
2726
|
|
Standard Deviation
|
13.6
|
49.5
|
86.4
|
52.1
|
120.9
|
151.2
|
|
CV%
|
2.3
|
3.5
|
3.6
|
7.8
|
7.7
|
5.5
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of sVCAM-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
