Human VEGF-D Quantikine ELISA Kit
Human VEGF-D Quantikine ELISA Kit
Human VEGF-D Quantikine ELISA Kit Summary
货号：DVED00
深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human VEGF-D
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human VEGF-D Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF-D levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed, recombinant human VEGF-D and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF-D showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF-D.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
560
|
1098
|
2192
|
512
|
978
|
1978
|
|
Standard Deviation
|
31.8
|
35.5
|
45.6
|
46.3
|
72.8
|
117
|
|
CV%
|
5.7
|
3.2
|
2.1
|
9
|
7.4
|
5.9
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
448
|
970
|
2081
|
474
|
956
|
2013
|
|
Standard Deviation
|
27.8
|
40.9
|
49.3
|
38
|
68.5
|
145
|
|
CV%
|
6.2
|
4.2
|
2.4
|
8
|
7.2
|
7.2
|
Recovery
The recovery of VEGF-D spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
100
|
97-104
|
|
EDTA Plasma (n=5)
|
105
|
89-114
|
|
Heparin Plasma (n=5)
|
104
|
88-114
|
|
Serum (n=5)
|
106
|
94-113
|
DVED00
