热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：MOST00
货号：96T
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
The recovery of mouse OPN spiked to three levels throughout the range of the assay was evaluated.
Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity
8.5 pg/mL
Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse and rat OPN. This assay cross-reacts 8.2% with recombinant Human Osteopontin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
62
67
59
Mean
96
236
1085
116
279
1076
Standard Deviation
7.3
13.8
43.4
9.8
22.5
90
CV%
7.6
5.8
4
8.4
8.1
8.4
Recovery
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Supernates (n=7)
98
87-106
Rat Cell Culture Supernates (n=7)
93
82-107
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