热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！
货号：MKM100
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
The recovery of TIM-1 spiked into various matrices was evaluated.
Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Sensitivity
2.18 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse TIM-1. The antibody components of this kit were raised against the short splice form of mouse TIM-1 (isoform 2) which has a 23 amino acid deletion following Pro182. This immunoassay detects both the short and long forms of mouse TIM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
24.2
61.8
207
23.2
60
190
Standard Deviation
0.8
1.6
6.8
2.5
4.2
13.2
CV%
3.3
2.6
3.3
10.8
7
6.9
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
100
91-106
EDTA Plasma (n=4)
89
88-91
Heparin Plasma (n=4)
87
86-89
Serum (n=4)
95
93-97
Urine (n=4)
102
94-115
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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