Human CXCL5/ENA-78 Quantikine ELISA Kit
Human CXCL5/ENA-78 Quantikine ELISA Kit
Human CXCL5/ENA-78 Quantikine ELISA Kit Summary
货号：DX000
规格: 96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human ENA-78
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human ENA-78 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human ENA-78 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human ENA-78 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human ENA-78 accurately. Results obtained using natural human ENA-78 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ENA-78.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
113
|
244
|
995
|
109
|
240
|
989
|
|
Standard Deviation
|
9.4
|
12.9
|
37.8
|
10.1
|
17.8
|
65.8
|
|
CV%
|
8.3
|
5.3
|
3.8
|
9.3
|
7.4
|
6.7
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
112
|
251
|
1096
|
129
|
278
|
1085
|
|
Standard Deviation
|
6
|
14.6
|
44.9
|
12.6
|
26.8
|
96.5
|
|
CV%
|
5.4
|
5.8
|
4.1
|
9.8
|
9.6
|
8.9
|
Recovery
The recovery of ENA-78 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
101
|
95-107
|
|
Citrate Plasma (n=5)
|
100
|
94-106
|
|
EDTA Plasma (n=5)
|
98
|
92-104
|
|
Heparin Plasma (n=5)
|
98
|
93-103
|
|
Serum (n=5)
|
101
|
93-109
|
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of ENA-78 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
