Human LIGHT/TNFSF14 Quantikine ELISA Kit
Human LIGHT/TNFSF14 Quantikine ELISA Kit
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Human LIGHT/TNFSF14 Quantikine ELISA Kit Summary
货号：DLIT00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human LIGHT
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human LIGHT Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LIGHT in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human LIGHT and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LIGHT showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LIGHT.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
350
|
712
|
1388
|
387
|
754
|
1469
|
|
Standard Deviation
|
12
|
31.7
|
63.3
|
19.6
|
31.8
|
63.4
|
|
CV%
|
3.4
|
4.5
|
4.6
|
5.1
|
4.2
|
4.3
|
Cell Culture Supernates, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
308
|
626
|
1181
|
318
|
616
|
1177
|
|
Standard Deviation
|
11.7
|
25.3
|
34.6
|
27
|
44.2
|
81.8
|
|
CV%
|
3.8
|
4
|
2.9
|
8.5
|
7.2
|
6.9
|
Recovery
The recovery of LIGHT spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
105
|
100-113
|
|
EDTA Plasma (n=5)
|
106
|
91-114
|
|
Heparin Plasma (n=5)
|
101
|
89-115
|
|
Serum (n=5)
|
102
|
85-114
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of LIGHT were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
