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梭菌蛋白酶(胞内蛋白酶-精氨酸-羧基)CAS : 9028-00-6
英文名: Clostripain (Endoproteinase-Arg-C)
货号:zk4323
别名: Endoproteinase-Arg-C
级别 :Activity ≥50 U/mg dw
储存 :冷藏(2-8℃)
规格:1mg 5mg
Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from?Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.
| Source | Clostridium histolyticum |
| Description | Chromatographically purified. A dialyzed, lyophilized pre-activated powder. |
| Storage | Store at 2-8°C |
| Activity | ≥50 units per mg dry weight |
| Unit Definition | One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25°C, pH 7.6, in the presence of dithiothreitol. |
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