热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
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0755-28715175
热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
中性蛋白酶，分散酶，已纯化CAS : 78990-62-2
货号：zk6118
英文名： Neutral Protease (Dispase), Purified
别名： Dispase; 分散酶
级别： Activity ≥4 U/mg dw
储存： 冷藏（2-8℃） 分子量： 36 kDa
规格：10mg 50mg 250mg
Neutral protease (Dispase?) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at our company. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.
| pH Optimum | Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0 |
| Stability | Stable at 2-8°C for 12 months. Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media |
| Specificity | Non-specific cleavage of peptide bonds containing leucine and phenylalanine. |
| Activators | Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2 |
| Inhibitors | EDTA (1mM), EGTA, 1-10-phenanthroline and heavy metals |
| Application | Commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application. |
| Source | Bacillus polymyxa |
| Description | Chromatographically purified. A lyophilized powder. |
| Storage | Store at 2-8°C. |
| Activity | ≥4 units per mg dry weight |
| Unit Definition | One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate. |
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