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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：MBLYS0
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of mouse BAFF/BLyS spiked to three levels throughout the range of the assay was evaluated.
Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (40 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL)
Sensitivity
7.8 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse BAFF/BLyS
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
50
64
56
Mean
125
288
1315
127
325
1262
Standard Deviation
3.6
7
30.3
16
27
136
CV%
2.9
2.4
2.3
12.6
8.3
10.8
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=6)
103
90-108
EDTA Plasma (n=6)
96
85-103
Heparin Plasma (n=6)
94
85-100
Serum (n=6)
98
86-115
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