Mouse Thrombopoietin Quantikine ELISA Kit
Mouse Thrombopoietin Quantikine ELISA Kit
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Mouse Thrombopoietin Quantikine ELISA Kit Summary
货号：MTP00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Citrate Plasma (10 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant mouse Tpo
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Tpo Immunoassay is a 4.5 hour solid phase ELISA designed to measure Tpo in cell culture supernates, serum, and plasma. It contains recombinant mouse Tpo and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Tpo showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Tpo.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
123
|
455
|
2364
|
119
|
457
|
2389
|
|
Standard Deviation
|
9.8
|
17
|
58.4
|
9.6
|
21.7
|
115
|
|
CV%
|
8
|
3.7
|
2.5
|
8.1
|
4.7
|
4.8
|
Recovery
The recovery of mouse Tpo spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
97
|
87-115
|
|
Citrate Plasma (n=5)
|
104
|
88-113
|
|
EDTA Plasma (n=5)
|
104
|
87-116
|
|
Serum (n=5)
|
100
|
88-111
|
